The following describes our wishes:
1. To juice about 1000 Kg of celery stalks at 4 C and neutralize with 60L
of buffer containing PMSF.
2. To perform a 25-80 % ammonium sulfate fractionation step at 4 C.
3. To dialyze the 80% ammonium sulfate pellet against a low salt buffer and
return the frozen solution to us.
Thanks!
Tony
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Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
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