hplc-systems

Gautam Sarath (gsarath@unlinfo.unl.edu)
Mon, 20 Jul 1998 16:43:35 -0500 (CDT)

Dear Corrado: I have used Waters HPLC systems for the last decade or so
and have found them to be very reliable and easy to maintain. However,
they are not too cheap nor is their software for double control. I have
switched to the BioCAD almost exclusively for routine purifications of
synthetic peptides and many proteins. This instrument is a workhorse if
maintained properly. It does have problems, but is no different than other
automated systems.

The UNL-Biochemistry Dept. recently purchased a Waters 625 system
with a PDA-detector. It has not yet been installed so I do not have data
on this beastie yet.

Most people I have spoken to, generally have a favorite
manufacturer and instrument type. My familarity with Pharmacia is with the
FPLC, this was suited to the job in the old days when HPLCs were made from
stainless steel. The new PEEK based instruments outperform FPLCs.

Hewlett Packard provided very poor response to our HPLC requests, A
local firm in town uses Rainin and is very content with the performance of
these machines. They are also easy to repair and good workhorses.
Finally, if you have the money, I like the BioCAD for purifications, the
analytical system is probably any one of the following three: Waters,
Rainin or Beckman. Gautam

Gautam Sarath
N-226, Beadle Center
Protein Core Facility - Center for Biotechnology &
Department of Biochemistry
University of Nebraska-Lincoln
Lincoln, NE 68588-0664
Phone: 402-472-2928
FAX: 402-472-7842
http://www.biotech.unl.edu/Proteins/index.html