Re: Isodityrosine
Dan Crimmins (crimmins@pathbox.wustl.edu)
Tue, 21 Jul 1998 08:14:15 -0500
At 01:38 PM 7/20/98 -0500, Lawrence J. Dangott wrote:
>Hi,
>
>Does anyone know a way to break isodityrosine bonds before loading a sample
>on a gel? Boiling alone does not do it. Is anyone aware of tyrosines being
>responsible for protein dimerization? In plant cell walls it is associated
>with crosslinking.
>
>Thanks for any ideas.
>
>Larry Dangott
>
>
>
>
>********************************************************
>Larry Dangott, Ph.D.
>Protein Chemistry Laboratory
>Texas A&M University
>College Station, TX 77842-3012
>(409) 845-2965
>
>
>
Larry,
Photolysis of Calmodulin (2 tyrosines) produces primarily intramolecular
dityrosine cross-links. Some intermolecular dimers are generated depending
on protein concentration, reaction conditions, etc. Sonja (?) Anderson has
published on this in Biochemistry and Analytical Biochemistry, I think in
the last 5 years.
Regards,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu