Di and trityrosines are also found in insect cuticles and in fertilization
membranes of echinoderms. The latter was studied in Ben Shapiro's lab
about 15 years ago. The synthesis of the C-C bond between the 2 rings is
promoted by peroxide. The product is very stable, eg it survives
hydrolysis of the proteins in 6N HCl to amino acids.
Ken
Kenneth A. Walsh
E.W.Davie/ZymoGenetics Chair of Biochemistry
Box # 357350
University of Washington
Seattle WA 98195
walsh@u.washington.edu
Phone 206-543-1768
FAX 206-685-9231
On Mon, 20 Jul 1998, Lawrence J. Dangott wrote:
> Hi,
>
> Does anyone know a way to break isodityrosine bonds before loading a sample
> on a gel? Boiling alone does not do it. Is anyone aware of tyrosines being
> responsible for protein dimerization? In plant cell walls it is associated
> with crosslinking.
>
> Thanks for any ideas.
>
> Larry Dangott
>
>
>
>
> ********************************************************
> Larry Dangott, Ph.D.
> Protein Chemistry Laboratory
> Texas A&M University
> College Station, TX 77842-3012
> (409) 845-2965
>
>