There are several tags you can use. High affinity ones include fluorescein
and anti-fluorescein (Molecular Probes, several versions), digoxigenin and
anti-digoxigenin (Boehringer Mannheim), and Bromo deoxyuracil (BrdU) and
anti-BrdU (I have forgotten the supliers).
Tony
At 09:48 AM 7/21/98 -0400, Winnell H. Newman wrote:
>Hello to all:
>
>I will try to explain what we need. We are trying to design a detection
>system for the following:
>
>We have oligonucleotides of various lengths that we are attaching to
>plates (or something) with a biotin group which has been added during
>chemical synthesis.
>
>We want to know what kinds of "groups" we can add during chemical
>synthesis that we can later detect on the oligo by using commercially
>available anitbodies.
>
>Hope this makes sense. I am the synthesis person, not the
>microbiologist...but can forward any suggestions to that person.
>
>Thanks for any leads you can give us.
>
>Sincerely,
>
>Winnell
>
>
>--
>Winnell H. Newman, Director, Nucleic Acids Facility (NAF)
>North Carolina State University, Department of Biochemistry
>128 Polk Hall, Box 7622
>Raleigh, NC 27695-7622
>
>Email = winnell@ncsu.edu
>VOX (919) 515-3463
>FAX (919) 515-2047
>
>
>
>
************************************
Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
************************************