After "in gel" digestion (trypsin) of an unknown protein
I measured the full MS spectra of the mixture of peptides with
Finnigan LCQ.
I obtained some small peaks and a wonderful "pattern" of 7 peaks.
Each peak is +14 compared to the previous.
In detail I have: 608.7 / 622.5 / 636.6 / 650.7 / 664.6 / 678.5 / 692.6
Moreover every peak is a pattern itself: three peaks which
the difference is +1, I guess due to the loss of a proton (?).
(eg: 650.7 / 651.7 / 652.6 intensity decreasing)
Do you have any idea of the origin of this pattern?
Maybe some contamination during the work?
Thanks in advance!
Federico
-- Federico Brianza Protein Laboratory Institute of Molecular Biotechnology Beutembergstr. 11, 07745 Jena / Germany Tel: +49 3641 656432 Fax: + 49 3641 656431