Peptide Content

Henry Wolfe (hnwo@americas.nycomed.com)
Wed, 22 Jul 1998 12:12:07 -0600

I am looking for a sensitive, linear, quantitative metod for determining
peptide content. The restrictions I am under are the following: I have a
12-residue peptide with one primary amine, three disulfides and no
aromatic amino acids. Also, I do not own an amino acid analyzer or
protein sequencer, but have good UV, fluorescence, CE and HPLC
equipment. Any suggestions? References to journal articles would be an
added bonus.

Thanks,

Hank Wolfe
henry.wolfe@nycomed-Amersham.com