Below you will find the complete list of papers describing SPPS-related
applications of fluorescamine. One of the protocols you may also find in
the SPPS manual by Stewart J.M. and Young J.D. (Solid phase
peptide synthesis.-Rockford, IL: Pierce Chem.Co.- 1984.)
Weigele M, DeBernardo S.L., Tengi J.P. and Leimgruber W.
A novel reagent for the fluorometric assay of primary amines.-
J. Am. Chem. Soc., 1972, v.94, N16, pp.5927-5928.
Udenfriend S., Stein S., Bohlen P., Dairman W., Leimgruber W. and
Weigle M.
Fluorescamine: A reagent for assay of amino acids, peptides, proteins,
and primary
amines in the picomole range. Science, 1972, v.178, N4063, pp.871-872.
Felix A.M. and Jimenez M.H.
Rapid fluorometric detection for completeness in solid phase coupling
reactions.
Anal. Biochem., 1973, v.52, N2, pp.377-381.
Tometsko A.M. and Vogelstein E.
Quality control during peptide synthesis. I. Quantitative monitoring
of resin
bound amines.- Anal. Biochem., 1975, v.64, N2, pp.438-443.
/fluram quantitation after cleavage of peptides with MeONa/
Tometsko A.M., Schreiner M. and Comstock J.
Resin probe analysis. II. Amino acid coupling reactions.
Anal. Biochem., 1975. v.67, N1, pp.182-191.
Smith J.A. Evaluation of the florescamine test for moni-
toring completion of coupling on nitrochloromethylated resin.-
Anal. Biochem., 1976, v.74, N2, pp. 477-483.
With all my best,
Sincerely,
Igor Rodionov
Laboratory Of Peptide Chemistry
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Pushchino, Moscow Region
142292, Russian Federation
E-mail: <rodionov@fibkh.serpukhov.su> or <Igor_Rodionov@iname.com>
Fax: (0967)-79-05-27
Phones: (0967) 73 54 42; private: (0967) 73 58 26