Calculation of Protein Extinction Coefficients from Amino Acid Sequence
Data Stan Gill and Pete von Hippel Analytical Biochemistry 182, 319-326
(1989). The molar extinction coefficient of cysteine is your key tool
here along with your UV spectrometer.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403
On Wed, 22 Jul 1998, Henry Wolfe wrote:
> I am looking for a sensitive, linear, quantitative metod for determining
> peptide content. The restrictions I am under are the following: I have a
> 12-residue peptide with one primary amine, three disulfides and no
> aromatic amino acids. Also, I do not own an amino acid analyzer or
> protein sequencer, but have good UV, fluorescence, CE and HPLC
> equipment. Any suggestions? References to journal articles would be an
> added bonus.
>
> Thanks,
>
> Hank Wolfe
> henry.wolfe@nycomed-Amersham.com
>