cys derivatization

Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Thu, 23 Jul 1998 10:01:00 +0000

We have just gotten an LCQ and have found it has a sensitivity sufficient
to analyze in-gel digests of proteins that are VERY WEAKLY silver stained
(i.e, we can use fluorescent stains or radiolabeled samples). This means
that the keratin contamination caused by reduction with DTT or
mercaptoethanol prior to alkylation is now a serious problem. What
alternative protocols and tricks are being tried out there in ABRF land to
step down in sensitivity for in-gel digests (untried suggestions are also
welcome)?

Katheryn Resing