Regards,
Rob Moritz.
PS. Don't have shares in Bio-Rad (not paid enough to have shares)
-----Original Message-----
From: Klein, Mike L. [mailto:mklein@amgen.com]
Sent: Friday, 24 July 1998 5:56
To: Robert Moritz
Subject: RE: Buying FPLC system
With respect to David's final paragraph, the HP1100 is NOT available
with titanium. I just spoke with HP reps a couple of days ago to
address the same issues, and their suggestion is to replace the
stainless steel tubing with PEEK and other parts with PEEK- or
Teflon-lined components. (If you go with the 1100 binary pump, they
have no replacement for the stainless steel mixer; you'd have to install
your own SS-free mixer.) I'm still trying to decide if this is the way
I should go, given our previous success with the 1100 for less salty
methods.
Mike Klein
Amgen, Inc.
> ----------
> From: schooley@unr.edu[SMTP:schooley@unr.edu]
> Sent: Thursday, July 23, 1998 10:39 AM
> To: Recipients of ABRF List
> Subject: Re: Buying FPLC system
>
> Dear Aki,
> To my knowledge FPLC is a trademark of Pharmacia and no one
> else
> can advertize an FPLC. However, as you intimate there are other
> instruments around that will do everything an FPLC will do, and more,
> at a
> lower price. I have two Perkin-Elmer 410 BIO systems, which have
> titanium
> pumps, injectors, tubing, and flow cells (instead of the glass
> cylinders in
> the low-medium pressure FPLC). They also have a flushable chamber
> (usually
> containing water) behind the pump seal, a very clever way of dealing
> with
> the problem of salt building up on the piston and damaging the pump
> seal.
> I have had these units over nine years; they require new pump seals
> about
> every other year. I believe they are the lowest maintenance LC's I
> have
> ever owned from any manufacturer (quite a list!). Plus they give you
> HPLC
> operation to 6,000 psi, unlike an FPLC, and they can be operated ina
> cold
> room. These instruments use low pressure, quaternary mixing, which
> is
> also a plus over the FPLC; EXCEPT that it introduces a 3 ml gradient
> dealy
> volume so they are not that useful for columns under 4.6 mm ID. THESE
> UNITS ARE DISCONTINUED, but I imagine that a newer P-E model has
> similar
> capabilities.
> For that matter, the H-P 1050, and presumably also the 1100,
> could also be purchased with Ti components to give you the inertness
> to
> salt that you get from Pharmacia.
>
> >Our lab is looking to buy an new FPLC, and like to get some
> >comments/recommendations on the FPLC systems on a market. I'd
> appreciate
> >if anyone could let me know about it. Thanks in advance.
> >
>
> David
>
> David A. Schooley
> Dept. of Biochemistry/330
> Univ. of Nevada
> Reno, NV 89557
> schooley@med.unr.edu
> tel: (702) 784-4136; fax (702) 784-1419
>
>