Sequencing PCR Products onthe abi377XL

robert obrien (rfob@email.msn.com)
Thu, 23 Jul 1998 22:20:39 -0400

I have recently begun sequencing 200bp pcr products on the 377XL and have
been noticing the following abnormalities: Peaks have a flat top appearance
to them and there are peaks under larger ones. Is it customary to get a lot
of N calls? What% is acceptable to get?

Any Suggestions will be greatly appreciated

Thank You

Robert O'Brien