RE: Sequencing PCR Products onthe abi377XL

Andy Ament (ament@genomesystems.com)
24 Jul 98 01:31:08 -0500

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Reply to: RE: Sequencing PCR Products onthe abi377XL
Robert,
No, it seems as if there is either something going on in your template =
prep or your cycle sequencing. Please supply me with a little more =
information. Are you using terminators or dye chemistry? Did you run new =
matrices with your pcr products? What vector and primers are you using? =
PCR products normally sequence as well if not better than plasmids.
-Andy
robert obrien wrote:
I have recently begun sequencing 200bp pcr products on the 377XL and have
been noticing the following abnormalities: Peaks have a flat top =
appearance
to them and there are peaks under larger ones. Is it customary to get a =
lot
of N calls? What% is acceptable to get?

Any Suggestions will be greatly appreciated

Thank You

Robert O'Brien

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From: "robert obrien" <rfob@email.msn.com>
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Subject: Sequencing PCR Products onthe abi377XL
Date: Thu, 23 Jul 1998 22:20:39 -0400
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         Reply to:   RE: Sequencing PCR Products onthe abi377XL
Robert,
No, it seems as if there = is either something going on in your template = prep or your cycle sequencing. Please supply = me with a little more information. Are = you using terminators or dye chemistry? = Did you run new matrices with your pcr products? = What vector and primers are you using? = PCR products normally sequence as well if = not better than plasmids.
-Andy
robert = obrien wrote:
I = have recently begun sequencing 200bp pcr = products on the 377XL and have
been noticing = the following abnormalities: Peaks have = a flat top appearance
to them and there = are peaks under larger ones. Is it customary = to get a lot
of N calls? What% is acceptable = to get?


Any Suggestions will be = greatly appreciated


Thank You

Robert = O'Brien





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Subject: Sequencing = PCR Products onthe abi377XL
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