Just a cautionary note on the G3's and Analysis 3.2. We bought the 96-lane
upgrade (also not installed yet) and a G3 computer from a local
distributor. The new G3 came with OS 8.1 extended (or plus). Analysis 3.2
is recommended for system 8. You cannot use the installer on Analysis 3.2
with OS 8.1 extended. PE-Biosystems recommended we down-grade to OS 8
(comes with the 3.2 software). Unfortunately, we can't. Apple tells us
that the newer G3's can't run OS 8, can only downgrade to 8.1 (plain). We
have managed to install A3.2 with OS8.1, but the crash occurs when
Analysis is set to automatically launch after data collection. I agree
with Hal, the tracker really works well, so it doesn't set us back too
much, but it is a shame that now there is finally an analysis version you
COULD reasonably set up for automatic analysis, that we CAN'T. We have
been talking with PE-Biosystems about this, and hopefully there will be a
fix, as those new G3's are out there. If anyone else has encountered this
problem and found a solution, please let me know.
At 12:36 AM -0500 7/24/98, Harold G. Hills wrote:
>Our 96 well upgrades have not been installed but I have been using the 3.2
>software with our 64 lane gels. The software is incredible in its ability
>to track gels especially for situations like ours in a core facilty where
>reactions often do not work. It even tracks gels which are distorted. In
>addition it generates analyzed data from very weak signals. They have
>added an interpolation button that adjusts all lanes between the two
>selected lanes. There are control points in the lanes already, no need to
>keep cutting the tracker lines to follow distorted lanes. The gels still
>need a bit of tweeking but its very easy to do.
>
>It takes about 25 minutes to track a 64 lane gel on my 8600/200 with 96 mb
>of Ram. I have not tried it on our G3's yet.
>
Mary Kay Dolejsi, Ph.D.
Manager, Biotech lab
marykay@fred.fhcrc.org
phone: 206-667-4470
fax: 206-667-6497
Mail address:
Fred Hutchinson Cancer Research Center
1100 Fairview Ave N. A1-162
PO Box 19024
Seattle, WA 98109-1024