Posttranslational Modifications

Kenneth Williams (Kenneth.Williams@yale.edu)
Mon, 27 Jul 1998 16:49:28 -0400

One of our users is working with a kinase that is phosphorylated at a
single site. In addition, however, the non-phosphorylated (and
dephosphorylated) kinase migrates as a doublet on isoelectric focussing -
with the distance between the two (non-phosphorylated) species being about
twice the distance between the phospho and non-phosho forms of either
individual species. My question is what are the most likely
post-translational modifications that might give rise to the original
doublet (assuming one species in not modified and that neiterh is
phosphorylated).

Thanks!


Ken Williams, Ph.D.
Director, HHMI Biopolymer/Keck Laboratory
Professor (Adjunct) Research
Molecular Biophysics and Biochemistry

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