Modified Met

Jacek_Mozdzanowski-1@sbphrd.com
Tue, 28 Jul 1998 16:44:56 -0400

To: abrf@aecom.yu.edu
cc:
From: Jacek Mozdzanowski-1 @ SB_PHARM_RD
Date: 28-Jul-98 09:44:56 PM
Subject: Modified Met
Categories:

Barb,

The easiest explanation may be provided if you reduced and
carboxymethylated the protein prior to analysis. If my guess about
reduction/carboxymethylation is correct, the peak is a peptide which
contains carboxymethylated/degraded methionine (mass decrease = 48 Da).
See Anal.Biochem. 216 (1994) 135-146.

Jacek Mozdzanowski
Bioanalytical Sciences
SmithKline Beecham