I have problem and looking forward for the solution. Can any one
kindly help me?
I have a 14 residue peptide which is highly hygroscopic. I want
to run the HPLC and purify it. It is insoluble in many solvents I tried
like ch3cn, dmso, h2o, isopropanol and the various ratios of their
mixtures. I also tried adding 1% TFA. I am unsuccessful.
It goes in CH3CN and water solution with TFA. Solubility
increases with addition of more TFA. Is it safe to use TFA and run HPLC
column? or is there any other solution?
Please advise.
Thanks
suryaprakash
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Dr. N. suryaprakash
department of chemistry
Ben Gurion University of the Negev
Beer Sheva
Israel
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