I'm unqualified to speak for the pepsin experts, but I do recall that
pepsin activates pepsinogen, so with a source of pepsinogen you could have
any level of sensitivity you want.
Ken
Kenneth A. Walsh
E.W.Davie/ZymoGenetics Chair of Biochemistry
Box # 357350
University of Washington
Seattle WA 98195
walsh@u.washington.edu
Phone 206-543-1768
FAX 206-685-9231
On Wed, 29 Jul 1998, Elizabeth Fowler wrote:
> Dear ABRFers:
>
> We are trying to develop an assay to detect a low level of pepsin activity
> in the presence of a large amount of protein. The approach of measuring
> production of TCA soluble peptides from hemoglobin is not sensitive enough.
> Does anyone know of a suitable colorimetric substrate, such as the BAEE
> assay for trypsin? Or have any other types of assays to suggest we look at?
>
> Many thanks,
>
> Beth Fowler
> Elizabeth Fowler
> Director, Biochemistry and Process Development
> AutoImmune Inc.
> 128 Spring Street
> Lexington, MA 02173
> Phone: 781-860-0710 x 230
> FAX: 781-860-0705
> Email: efowler@ma.ultranet.com
>
>