It depends on how much TFA you're going to have to use and what sort of
column you're loading onto. What scale are you working on? I have, in the
past, used neat TFA to dssolve particularly troublesome peptides for
analysis and/or purification. I have routinely used guard columns on both
analytical and preparative columns to try to protect them from any cleavage
of C18, but with fully derivatized and end-capped columns, I haven't had
any problems. I think the odd one-off "hit" of neat TFA (or high conc. of
TFA in water and/or organic) would be okay. You can always run a standard
afterwards to make sure you still have a viable column.
Hope this helps,
Roger
At 12:50 PM 29-07-98 +0300, you wrote:
>
>Hai Everyone
>
> I have problem and looking forward for the solution. Can any one
>kindly help me?
>
> I have a 14 residue peptide which is highly hygroscopic. I want
>to run the HPLC and purify it. It is insoluble in many solvents I tried
>like ch3cn, dmso, h2o, isopropanol and the various ratios of their
>mixtures. I also tried adding 1% TFA. I am unsuccessful.
>
> It goes in CH3CN and water solution with TFA. Solubility
>increases with addition of more TFA. Is it safe to use TFA and run HPLC
>column? or is there any other solution?
>
> Please advise.
>
> Thanks
> suryaprakash
>
>
>---------------------------------------------------------------------------
>
>Dr. N. suryaprakash
>department of chemistry
>Ben Gurion University of the Negev
>Beer Sheva
>Israel
>---------------------------------------------------------------------------
>
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email murphy_r@licre.ludwig.edu.au