I don't remember who was advertising what at ABRF, but I have used three
different columns that gave good results with a TFA free system (HAc and
HFBA instead of TFA). These were:
MS Magic from Michrom BioResources (916) 888-6498
Monitor from Column Engineering (800) 707-4752
Targa from Higgins Analytical (650) 988-8930
I have done most of my work with the MS Magic material, and found it works
well for most proteins and peptide maps. As with most RP-HPLC if you pick
the right protein you can make any one of these columns look better than
the others.
Hope this helps
--tks
Tim Slattery
Protein Biochemistry and Biophysics Department
Berlex Biosciences
tim_slattery@berlex.com
______________________________ Reply Separator _________________________________
Subject: TFA free HPLC
Author: Ken Mitchelhill <k.mitchelhill@medicine.unimelb.edu.au> at Internet
Date: 7/31/98 10:42 AM
Colleagues,
my (poor) memory is that a vendor was promoting some reversed phase columns
at ABRF98 that didn't require TFA. The chromatograms that were displayed
were pretty nice looking (although vendor chromatograms usually are).
I didn't get any information at the meeting was wondering who that vendor
was and if anyone out there had any experience with these columns?
Regards...Ken
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Ken I. Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute of Medical Research
41 Victoria Parade
Fitzroy 3065 Victoria
AUSTRALIA
Telephone: 61-3-9288 2480
Facsimile: 61-3-9416 2676
Email: k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
ABRF: http://www.abrf.org
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