Have these alternative columns been useful for optimizing On-line LC/MS
analyses of proteins as well as peptide maps? I have been trying various
alternative mobile phases and separation modes to improve separation and MW
determination of intact proteins, but above 40-50kDA, the results are
mixed. Any tricks to it?
Alex Apffel, Ph.D.
Biomeasurements Group
Hewlett-Packard Laboratories
3500 Deer Creek Road
Palo Alto, CA 94304 USA
Tel 650-857-6090
Fax 650-852-8502
email alex_apffel@hpl.hp.com
> -----Original Message-----
> From: Tim_Slattery@berlex.com [mailto:Tim_Slattery@berlex.com]
> Sent: Friday, July 31, 1998 9:43 AM
> To: Recipients of ABRF List
> Subject: Re: TFA free HPLC
>
>
> Ken,
>
> I don't remember who was advertising what at ABRF, but I have
> used three
> different columns that gave good results with a TFA free
> system (HAc and
> HFBA instead of TFA). These were:
>
> MS Magic from Michrom BioResources (916) 888-6498
> Monitor from Column Engineering (800) 707-4752
> Targa from Higgins Analytical (650) 988-8930
>
> I have done most of my work with the MS Magic material, and
> found it works
> well for most proteins and peptide maps. As with most RP-HPLC
> if you pick
> the right protein you can make any one of these columns look
> better than
> the others.
>
> Hope this helps
>
> --tks
> Tim Slattery
> Protein Biochemistry and Biophysics Department
> Berlex Biosciences
> tim_slattery@berlex.com
>
>
> ______________________________ Reply Separator
> _________________________________
> Subject: TFA free HPLC
> Author: Ken Mitchelhill
> <k.mitchelhill@medicine.unimelb.edu.au> at Internet
> Date: 7/31/98 10:42 AM
>
>
> Colleagues,
>
> my (poor) memory is that a vendor was promoting some reversed
> phase columns
> at ABRF98 that didn't require TFA. The chromatograms that
> were displayed
> were pretty nice looking (although vendor chromatograms usually are).
>
> I didn't get any information at the meeting was wondering who
> that vendor
> was and if anyone out there had any experience with these columns?
>
> Regards...Ken
>
> ********************************
>
> Ken I. Mitchelhill
> The John Holt Protein Structure Laboratory
> St. Vincent's Institute of Medical Research
> 41 Victoria Parade
> Fitzroy 3065 Victoria
> AUSTRALIA
>
> Telephone: 61-3-9288 2480
> Facsimile: 61-3-9416 2676
>
> Email: k.mitchelhill@medicine.unimelb.edu.au
>
> Laboratory:
> http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRd> ocs/JHPSL.html
>
> ABRF: http://www.abrf.org
>
>
> ***********************************
>
>
>