Ben, I do not know a reference to a current kit, but I have some older
references that worked well, well, 20 years ago when I used them.
--After lipid extraction: E.G. Bligh and W.J. Dyer A Rapid Method of
Total Lipid Extraction and Purification, Can J Biochem and Phys 37, 911
(1959)
M.Kates "Techniques of Lipidology" North Holland Publishing Co.,
Amsterdam, The Netherlands, 1972
--Lipids were run on 2-D thin layer chomatography: J.G.Parsons and
S.Patton Two-dimensional thin-layer chromatography of polar lipids from
milk and mammary tissue J Lipid Res 8, 696 (1967)
G Rouser and S Fleischer Isolation, Characterization and
Determination of Polar Lipids of Mitochondria in Methods in Enzymology (I
have the page number, 385, but no volume--probably in 1966)
--Phospholipids are then visualized on the 2-D TLC by molybdenum blue
spray, see J.C. Dittmer and R.L. Lester J. Lipid Res. 5:126 (1964) (A
modification of the spray is outlined in K Kahma, J Brotherus, M Haltia and
O Renkonen Low and Moderate Concentrations of Lysobisphosphatidic Aid in
Brain and Liver of Patients Affected by Some Storage Diseases Lipids 11,
539 (1976)
--or phospholipids can be visualized by Iodine vapour.
If this is too ancient for you, try a search on othe Web! I have found
that extremely useful.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403