Antibodies to ribosomal proteins

VERNON SHOUP (vernon.shoup@regpha.com)
11 Aug 98 12:40:52 -0400

We are producing an E.coli-derived recombinant protein drug candidate that has a pI of about 10. Because of this high pI (positive charge under physiological conditions), ribosomal proteins tend to copurify with it. Eventually a host cell protein assay that will detect these proteins will be needed. We plan to send some empty vector material part way through the purification process to isolate the proteins, but in the interim...

We're interested in obtaining either some purified E.coli ribosomes, or more preferably the set of proteins isolated from the ribosomes. Analytical quantities would be OK for now. Does anyone know where I can obtain this material?
To push this a bit more, we'd also love to get hold of some polyclonal antibodies that would recognize a range of E.coli ribosomal proteins.
Does anybody have any suggestions? If these materials are available privately, we'd be glad to arrange payment.

Vernon

Vernon A. Shoup
Regeneron Pharmaceuticals, QC Dept.
81 Columbia Tnpk
Rensselaer, NY 12144

(518)488-6012
(518)488-6030 FAX
vernon.shoup@regpha.com