Tony
At 12:40 PM 8/11/98 -0400, VERNON SHOUP wrote:
>
>We are producing an E.coli-derived recombinant protein drug candidate that
has a pI of about 10. Because of this high pI (positive charge under
physiological conditions), ribosomal proteins tend to copurify with it.
Eventually a host cell protein assay that will detect these proteins will
be needed. We plan to send some empty vector material part way through the
purification process to isolate the proteins, but in the interim...
>
>We're interested in obtaining either some purified E.coli ribosomes, or
more preferably the set of proteins isolated from the ribosomes.
Analytical quantities would be OK for now. Does anyone know where I can
obtain this material?
>To push this a bit more, we'd also love to get hold of some polyclonal
antibodies that would recognize a range of E.coli ribosomal proteins.
>Does anybody have any suggestions? If these materials are available
privately, we'd be glad to arrange payment.
>
>Vernon
>
>Vernon A. Shoup
>Regeneron Pharmaceuticals, QC Dept.
>81 Columbia Tnpk
>Rensselaer, NY 12144
>
>(518)488-6012
>(518)488-6030 FAX
>vernon.shoup@regpha.com
>
>
>
************************************
Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
************************************