LYS-C:
We have generally observed 1-3 peaks that are lys-c related in our
HPLC chromatograms (3 if the enzyme has a higher amount of degradation).
The mass ULF reported as 1919.9 we definitely observe and we also see the
CAM-Cys form (+57 daltons) when we do reduction alkylation. Apparently
after alkylation there is some residual iodoacetamide remaining that
alkylates the cys. We have confirmd this by accidently sequencing both
derived forms of this peptide (which elute at different positions on the
HPLC).
The list we have is the same that ULF has, however we have
calculated slightly different masses using PROWL and SHERPA programs (we
are reporting the average mass, and not MH+)
1-30 3094.48
31-49 1922.11
50-106 5917.53
107-155 5503.05
156-203 4875.26
204-268 6514.00
Hope this helps
Joe
Joseph Fernandez
Associate Director
The Rockefeller university
Protein/DNA Technology Center
1230 York Ave. New York, NY 10021
Phone: (212)-327-8869
FAX : (212)-327-8620
email: fernaj@rockvax.rockefeller.edu
Lab Web Page: http:\\pdtc.rockefeller.edu