protein quantification

Helene Hill (hill@umdnj.edu)
Thu, 13 Aug 1998 14:38:57 -0400 (EDT)

I am purifying two proteins that are quite close on gels. I stain the gel
with coomassie, cut out the proteins and electroelute. I would like to
measure protein content on the eluate but most assays are based on
coomassie so they won't work. Can anyone tell me if the BCA assay would
do it? We have used an assay that depends on the size of the protein spot
on a membrane and find it is not very reliable.

I have tried staining the gel with copper but the bands are difficult to
distinguish. Would zinc stain be better?

Thank you.

Helene Z Hill, Ph.D. Internet: hill@umdnj.edu
Head, Section of Cancer Biology Voice/fax: (973)972-3421
Mail: MSB-E586
NJ Medical School
185 South Orange Ave
Newark, NJ 07103-2714