Re: gel electrophoresis of CNBr fragments

Axel Ducret (axel_ducret@merck.com)
Fri, 14 Aug 1998 09:06:11 -0400

Dear Deb,

Look in "Advanced Methods in Protein Microsequence Analysis" edited by B.
Wittmann-Liebold, J. Salnikow, V.A. Erdmann (1987) for an article written by
Gerhard Frank for a comprehensive protocol for gas-phase CNBr cleavage on PVDF
membranes and subsequent analysis of the fragments by SDS-PAGE.

Good luck

Axel

--
Axel Ducret, Ph.D.
Senior Research Biologist
Merck-Frosst Canada Inc.
Dept. Biochemistry and Molecular Biology
P.O. Box 1005
Pointe-Claire-Dorval PQ H9R 4P8
Canada

tel. + (514) 428-3428 fax + (514) 428-4900