Look in "Advanced Methods in Protein Microsequence Analysis" edited by B.
Wittmann-Liebold, J. Salnikow, V.A. Erdmann (1987) for an article written by
Gerhard Frank for a comprehensive protocol for gas-phase CNBr cleavage on PVDF
membranes and subsequent analysis of the fragments by SDS-PAGE.
Good luck
Axel
-- Axel Ducret, Ph.D. Senior Research Biologist Merck-Frosst Canada Inc. Dept. Biochemistry and Molecular Biology P.O. Box 1005 Pointe-Claire-Dorval PQ H9R 4P8 Canadatel. + (514) 428-3428 fax + (514) 428-4900