Jelle
____________________________________________________
Jelle Lahnstein
Nucleic acid and Protein Chemistry Unit
Department of Plant Science
Waite Campus, University of Adelaide
Glen Osmond, 5064, Australia
Tel : 61 - 8 - 83037260
Fax : 61 - 8 - 83037130
Internet : http://www.waite.adelaide.edu.au/NAPCU/
On Fri, 14 Aug 1998, Edmunds, Tim wrote:
> A word of caution.
>
> There are two proteases commonly used for cleavage at Lys residues.
> Wako sells Lysyl Endopeptidase from Achromobacter lyticus E.C.
> 3.4.21.50. The enzyme from Boehringer is Endoproteinase Lys-C from
> Lysobacter enzymogenes E.C.3.4.99.30. Unfortunately, for convenience,
> both of these proteinases are often called Lys-C. When looking for
> autolytic fragments it is obviously important to make sure you are
> trying to match masses to the correct sequence.
>
> I know the general consensus has been that the Wako enzyme gives more
> reproducible results but we have not found that to be true for the
> proteins we have digested. We switched from the Wako enzyme to the
> Boehringer sequencing grade enzyme many years ago due to a high
> background of autolytic fragments and incomplete digestion products
> obtained with the Wako enzyme. Obviously there is a significant cost
> difference between the Boehringer sequencing grade enzyme and the Wako
> enzyme and our observations may not hold up for the Boehringer bulk
> Lys-C.
>
> Tim
>
> Tim Edmunds
> Director
> Structural Protein Chemistry
> Genzyme Corporation
> P.O Box 9322
> Framingham
> MA 01701-9322
>
> Tel (508) 270 2260
> Fax (508)872 9080
> EMail tedmunds@genzyme.com
>
>