Re: Endoproteinase Glu-C

Dan Crimmins (crimmins@pathbox.wustl.edu)
Tue, 18 Aug 1998 09:34:18 -0500

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At 08:44 AM 8/13/98 -0700, Christoph Turck wrote:
>
> Does anybody know if Endoproteinase Glu-C (EC 3.4.21.19) will cleave
>after glutamic acid residues that are flanked by phosphotyrosine on either
>side (...-pY-E-... or ...-E-pY-...)? We are using the enzyme for mapping
>tyrosine phosphorylation sites by radioactive Edman degradation.
>
>Thanks,
>
>Chris (turck@itsa.ucsf.edu)
>
>
>

Chris,
Look at Fig. 6A from Sun, X.J., Crimmins, D.L., Myers, M.G., Miralpeix,
M. and White, M.F. (1993) Molecular and Cellular Biology, 7418-7428.
"Pleitropic Insulin Signals are Engaged by Multisite Phosphorylation of IRS-1"

Best regards,

Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu

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