I've had a lot of success with the Dde group (removed by 2% hydrazine/DMF)
for orthogonal protection of lysine. It's very stable to most other things
and comes off easily with the hydrazine, just like they say in the
Novabiochem catalogue!
Good luck,
Roger
At 02:05 PM 19-08-98 -0700, you wrote:
>George and others-
>
> We have a client who wants a peptide synthesized with two Lys
>separated by Gly spacers, each to be tagged with a different fluorophor.
>I know little about orthogonal protection beyond the Novabiochem catalog.
>Is the Alloc group suitable for this? Is the deprotection similar to that
>of Allyl Asp or Glu given in the Nova catalogue, and is this the procedure
>of Barany mentioned in a reference we do not have easy access to??
> We are doing FMOC syntheses. Lastly, anyone have a good general
>reference to orthogonal protection strategies for FMOC synthesis?
> Thanks in advance for your help!
>
>David
>
>David A. Schooley
>Dept. of Biochemistry/330
>Univ. of Nevada
>Reno, NV 89557
>schooley@med.unr.edu
>tel: (702) 784-4136; fax (702) 784-1419
>
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email murphy_r@licre.ludwig.edu.au