Re: PSeq: His-tagged samples, a general question.

Frank_Masiarz@cc.chiron.com
Wed, 26 Aug 1998 13:43:26 -0700


Hi.................

Best thing to do: request location of the His tag at the C-terminus of
the protein.

Good Luck !!

Frank R. Masiarz
Protein Structure Analysis Group
Chiron Corporation

______________________________ Reply Separator _________________________________
Subject: PSeq: His-tagged samples, a general question.
Author: Thomas Miller <millertj@esvax.es.dupont.com> at SMTP
Date: 8/26/98 12:56 PM

Hello. I started receiving His tagged samples for protein
sequencing. The problem that I have seen is getting past
the tagged region. Should I be considering enzyme digests
in order to help characterize these samples? Any comments
are appreciated. Thank you. Regards, Tom Miller.


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** Thomas Miller, DuPont Co. **
** Voice: 302-695-1745 **
** Thomas.J.Miller@usa.dupont.com **
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