My facility has been doing HPLC quantitative analysis of the levels of
various nucleotides from tissue cultured cells over the last year or so and
are currently set up to run. You may need two gradients to resolve all the
nucleotides. GTP and GDP is the area of our interest. GDP is challenging
and requires spiking to identify. There are much details to talk about. If
interested, please call Tony Pomenti at 215-728-2489. He has been
instrumental to the development of the whole analysis procedure.
Tony
At 08:21 AM 8/27/98 +1000, Ken Mitchelhill wrote:
>Colleagues,
>
>can anyone help with the following query from a visitor to the ABRF Web site?
>
>Regards....Ken
>
>>Date: Wed, 26 Aug 1998 12:48:06 -0600
>>From: Owen Lockerbie <olockerbie@pharmadigm.com>
>>Subject: (no subject)
>>To: webmaster@abrf.org
>>MIME-version: 1.0
>>
>>do you know of a resource that will do HPLC analysis of guanine
>>nucleotides (GTP/GDP) in crude cellular mixtures
>>
>>owen lockerbie
>>
>
>********************************
>
>Ken I. Mitchelhill
>The John Holt Protein Structure Laboratory
>St. Vincent's Institute of Medical Research
>41 Victoria Parade
>Fitzroy 3065 Victoria
>AUSTRALIA
>
>Telephone: 61-3-9288 2480
>Facsimile: 61-3-9416 2676
>
>Email: k.mitchelhill@medicine.unimelb.edu.au
>
>Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
>ABRF: http://www.abrf.org
>
>***********************************
>
>
>
>
************************************
Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
************************************