> Hello. I started receiving His tagged samples for protein
> sequencing. The problem that I have seen is getting past
> the tagged region. Should I be considering enzyme digests
> in order to help characterize these samples? Any comments
> are appreciated. Thank you. Regards, Tom Miller.
>
>
>
Tom,
Like Joe we have no problems in sequencing His tagged proteins(normally H6),and
these are done on our 477A.
Sandy.
----------------------
Alex(Sandy) Carne,
Head of Protein Characterization,
Institute of Cancer Research,
Chester Beatty Labs.,
237 Fulham Road,
London SW3 6JB, UK.
Tel: +44 (0)171 352 8133
Fax: +44 (0)171 352 3299
E-mail: sandyc@icr.ac.uk
Mobile: 0370 365 032