I am trying to modify a peptide to serve as an internal standard for an
HPLC based assay. The requirement is that it elute in the very hydrophobic
range, say between 50 and 60% acetonitrile from a C8 column, as well as a
number of other structural features.
I have the base peptide on resin and I have tried a series of N-terminal
modifications, the most hydrophobic of which is myristoylation (C14) but
even the myristoyl-form only elutes at just over 40% acetonitrile.
I had thought about just going through the Sigma catalog and ordering a
series of longer alkyl chlorides but I thought I might ask if anyone has
experience of making this type of peptide and what the expected increase in
retention time might be for the addition of more methyl groups to the
N-terminus?
Regards....Ken
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Ken I. Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute of Medical Research
41 Victoria Parade
Fitzroy 3065 Victoria
AUSTRALIA
Telephone: 61-3-9288 2480
Facsimile: 61-3-9416 2676
Email: k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
ABRF: http://www.abrf.org
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