RE: lipoprotein

Apffel, Alex (apffel@exch.hpl.hp.com)
Thu, 27 Aug 1998 18:08:45 -0700

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Hi Katheryn,
Funny you should ask. I'm working on a project to optimize conditions for
on-line LC-ESI/MS of Largish Intact Proteins. And the next study (probably
next week) will be to try HILIC on a PolyLC PolyHydroxyethyl Aspartamide
column. It looks like an attractive combination since the mobile phases can
be volatile and relatively ESI-friendly. Furthermore, the same type of
column can be used in a denaturing SEC mode using 50mM Formic Acid as a
mobile phase. I guess Andy Alpert at PolyLC (410-992-5400)will have the
most up to date info on MS applications. I'll be interested in what other
response you get and any experiences you have with the technique.

Alex Apffel, Ph.D.
Biomeasurements Group
Hewlett-Packard Laboratories
3500 Deer Creek Road
Palo Alto, CA 94304 USA
Tel 650-857-6090
Fax 650-852-8502
email alex_apffel@hpl.hp.com

> -----Original Message-----
> From: Katheryn.Resing@Colorado.EDU
> [mailto:Katheryn.Resing@Colorado.EDU]
> Sent: Thursday, August 27, 1998 10:27 AM
> To: Recipients of ABRF List
> Subject: Re: lipoprotein
>
>
> Has anyone mated HILIC to ESI/MS.
>
> Katheryn Resing
>
>

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