Re: PSeq: His-tagged samples, a general question.

L. Ericsson (ericsson@u.washington.edu)
Sun, 30 Aug 1998 06:47:44 -0700 (PDT)

Most of the His-tagged samples we receive have a built in cleavage site
for enterokinase. Check with you client and see if they can make this
digest for you before you start the sequencing. Good idea to check
molecular weights by mass spec before and after cleavage.
-Lowell H. Ericsson, Dept. of Biochemistry, U. of Washington, Seattle, WA

On Wed, 26 Aug 1998, Thomas Miller wrote:

> Hello. I started receiving His tagged samples for protein
> sequencing. The problem that I have seen is getting past
> the tagged region. Should I be considering enzyme digests
> in order to help characterize these samples? Any comments
> are appreciated. Thank you. Regards, Tom Miller.
>
>
> ******************************************************
> ** Thomas Miller, DuPont Co. **
> ** Voice: 302-695-1745 **
> ** Thomas.J.Miller@usa.dupont.com **
> ** Opinions are my own and not of my employer. **
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