Re: HPLC

Igor Rodionov (rodionov@fibkh.serpukhov.su)
Mon, 31 Aug 1998 21:33:47 +0400

Dear Laurent,
oligo(Ala)-X have been extensively studied in the papers listed below but
I'm not sure if HPLC conditions could be found in them. RP HPLC is not a
method of choice for these strongly aggregating peptides.

H-(Ala)n-Val
Merrifield, R.B., Singer, J., and Chait, B.T. Mass Spectrometric
Evaluation of Synthetic Peptides for Deletions and Insertions.- Analytical
Biochem., 1988, v.174, pp.399-414.

H-(Ala)n-Phe
Thaler, A., Seebach, D., and Cardinaux, F. Improvement of Degree of Resin
Swelling and Efficiency of Coupling in Solid-Phase Synthesis.- Helvetica
Chimica Acta., 1991, v.74, pp.628-643.
Thaler, A., Seebach, D., and Cardinaux, F. Conditions for the Use of
Lithium-Salts in Coupling Reactions. Helvetica Chimica Acta, 1991, v.74,
pp.617-627.

H-(Ala)n-Lys
Larsen, B.D., Chistensen, D.H., Holm, A., Zillmer, R., and Faurskov
Nielsen, O., The Merrifield peptide synthesis studied by near-infrared
fourier-transform Raman spectroscopy.- J. Am. Chem. Soc., 1993, v.115, n.14
pp.6247-6253.

H-(Leu)n-Phe
Narita, M., Isokawa, S., Tomotake, Y., and Nagasawa, S. Synthesis and the
Solid-State Conformations of Cross-Linked Resin-Bound Oligo(leucine)s.
Internal Aggregations through the Intermolecular Hydrogen Bonding of the
Oligopeptides on the Polymer Networks.- Polymer Journal, 1983, v.15, n.1,
pp.25-32.

Hope this information will be useful

Regards
Igor

Laboratory of Peptide Chemistry
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Pushchino, Moscow Region
142292, Russian Federation
E-mail: <rodionov@fibkh.serpukhov.su> or <Igor_Rodionov@iname.com>
Fax: (0967)-79-05-27
Phones: (0967) 73 54 42; private: (0967) 73 58 26