References to ESMS and ESMS-MS for identification of glycerophospholipids:
J. Lipid Res. (1994) 35: 1102-1114.
Microbiology (1995) 141: 399-410.
J. Microbiol. Methods (1997) 28: 187-199.
PNAS (1994) 91: 10635-10639.
There are at least a dozen related articles published in the last 2-3
years if these aren't sufficient.
Specific reference for lysophospholipids:
J. Amer. Chem. Soc. (1996) 118-457.
Several laboratories have used chloroform/methanol (1/2, v/v) for infusion
of their GPL samples. We have found that more consistent results are
obtained with a maximum of 1-2% chloroform. The sensitivity of ESMS
during GPL analysis is such that, although these compounds are much more
soluble in CHCl3, MeOH is the preferred solvent.
You will encounter problems with quantitation unless you have a stable
isotope internal standard. Regards,
Jim Kerwin
Botany Department
University of Washington
Seattle, WA 98195
On Tue, 8 Sep 1998, Dr K.S. Lilley wrote:
> Dear All,
>
> one of my users wants to set up an mass spec. assay to measure lysophosphatidic
> acids (LPA) in urine. Has anyone got experience of running glycerophospholipids
> in electrospray?
>
> Thanks in advance
>
> Kathryn Lilley
>
> K.S.Lilley
> Laboratory Manager
> PNACL
> University of Leicester
> Lancaster Rd
> Leicester LE1 9HN
> UK
>
> email: ksl@le.ac.uk
> tel: 44 116 252 5613
> fax: 44 116 252 5616
>
>