Trypic digests on PVDF

Suzanne Perry-Riehm (perry@interchange.ubc.ca)
Tue, 8 Sep 1998 15:45:13 -0700 (PDT)

Has anyone ever attempted to "re-digest" a sample on PVDF that has been
previously reduced, alkylated and trypsin digested? I have experienced a
failure
of 3 different samples to yield any peptides but upon re-testing of the
trypsin found
the enzyme to be active. I still have the original PVDF pieces from the 3
samples in their original reaction tubes sitting on my lab bench.
I know this may be asking for a "Lazarus technique" but I am willing to
try it.

Thanks in advance.

Suzanne C. Perry, Research Associate
NAPS Protein Service Laboratory
Biotechnology Department, University of British Columbia
CANADA
phone:(604)822-9662 Fax:(604)822-0676 email:perry@unixg.ubc.ca
Visit our web page at : www.biotech.ubc.ca/naps/napshome.htm