Nanospray on the LCQ

Tim Slattery (Tim_Slattery@berlex.com)
Wed, 09 Sep 1998 10:10 -0800

I've had my LCQ for a year now, and would like to start working with
nanospray. At this point I have no experience and little information
about setting up a nano-electrospray system. If anyone could give me
the answers to a few questions, and maybe more importantly answer the
questions I don't have the experience to ask yet...

Does anyone make a commercial nano-electrospray attachment?

If you built one, were there any key references you used, or what are
the key points a first timer is likely to miss.

Do you buy tips, or pull your own?

What kind of flow rates and spray voltages do you use?

Do you put the tip into the heated capillary, or just keep a little
separation?


Thank you for any answers you can give.

--tks

Tim Slattery
Protein Biochemistry and Biophysics Department
Berlex Biosciences
tim_slattery@berlex.com