Don Sheer and antibody removal

Gary Hathaway (hathaway@cco.caltech.edu)
Thu, 10 Sep 1998 21:32:00 -0700

Ken,
Very sad news about Don. I knew him for many years since he was involved
with the development of the blot cartridge for ABI. As I recall, he also
was closely involved with the development of the prospin cartridge and
spent considerable time helping me set up my own "homespun" version long
before it was commercially available. That's the kind of guy he was. Don
also developed the SCX cartridge (I believe at Amicon). Anyone who has used
these technologies owes a debt of gratitude to Don Sheer. He was truely a
gentleman scientist and I will miss him.

re: your antibody problem
I haven't worked with antibodies for some time, but as I recall the
classical means to purify IgG was by DEAE chromatography. I couldn't tell
from your posting whether you are after the Ab or the antigen so whether or
not DEAE will work would depend.
Greybeard in Pasadena

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Gary M. Hathaway, Director
PPMAL - Protein/Peptide Micro Analytical Laboratory
California Institute of Technology
139-74, Division of Biology
Pasadena, CA 91125

http://www.cco.caltech.edu/~ppmal
email Gary: hathaway@caltech.edu
email facility: ppmal@caltech.edu
phone: lab (818) 395-6388 or office (818) 395-2769
FAX (818) 449-3414
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