The DMF and peperidine accentuated the problem are likely because of increased
absorption through the skin. I use ABI's suggestion on buying in bottles that
don't have to be split or weighed out. Just add the solvent directly into the
bottle without weight the material out. Anything that will happen with HBTU, is
likely to be reproduced with EDC, DCC, etc.
What's a few dollars of chemicals when compared to your health and the your
personnel.
David Chin
Usual disclaimer apply.
-----Original Message-----
From: Gautam Sarath <gsarath@unlinfo.unl.edu> at MU-Internet
Sent: Thursday, September 10, 1998 12:43 PM
To: abrf@aecom.yu.edu at MU-Internet
Cc: Chin, David T.
Subject: Peptide Synthesis/ HBTU allergic reaction.
Dear Steve: I get a mild rash when I come in direct contact with HBTU, but
I have thankfully not developed further reactions. I normally scrub
exposed skin areas down and rinse off balances etc after weighing HBTU.
Weighing HBTU in a hood might make life more comfortable for the person
with the allergic response. I also think that both DMF and piperidine
accentuate this problem, although my n=1. Reagrds, Gautam
Gautam Sarath
N-226, Beadle Center
Protein Core Facility - Center for Biotechnology &
Department of Biochemistry
University of Nebraska-Lincoln
Lincoln, NE 68588-0664
Phone: 402-472-2928
FAX: 402-472-7842
http://www.biotech.unl.edu/Proteins/index.html