We are having problems with 'ghost' -peaks that are appearing in are RPC
runs. We are using a spheri-5 rp-18 column with a precolumn ( rp-8)
The buffers are:
A: 0.1M Triethylamine (TEA, >99%) in HPLC-grade water brought to pH7 with
acetic acid (>99.8%)
B: 80% acetonitrile in water
INITIAL: 100% A
LINEAR GRADIENT to: 50/50 A/B
RINSE: 100% B
END: 100%A
We have tried many things to locate our problem. We changed the buffers,
the columns but still there is a clear peak ones the gradient is started.
It seems that this is a component that is present in the buffer A because
the peak increases with the volume of buffer A that was pumped over the
column. Could the stability of the triethylamine be given problems and
should we buy a new bottle since the present one is in use for some time
now?
Kind regards
Ben Van der Perre
benvdp@innogenetics.be
Immunochemistry
Innogenetics.