Ghost peaks
Stephen A. Bobin (Stephen.A.Bobin@Dartmouth.EDU)
28 Sep 98 16:53:07 EDT
Ben,
I believe you are right about the ghost being in buffer A. Try using some water from another source to make your buffer. I keep HPLC grade water from Fisher or B&J around in the lab just for such occasions. I worry about triethylamine, I usually assume it has gone bad the moment I open the bottle. A new bottle would be a good idea. Also, how long has that bottle of acetic acid been around? Jeez, I just looked at mine it has an opened date of May 10, 1995. I don't think it "goes bad" but I do think about all the plastic that has been dipped in it over three years.
Hope this helps.
Steve
Stephen A. Bobin
Manager
Molecular Biology Core Facility
Dartmouth College
Hanover, NH 03755