>It seems that this is a component that is present in the buffer A because
>the peak increases with the volume of buffer A that was pumped over the
>column. Could the stability of the triethylamine be given problems and
>should we buy a new bottle since the present one is in use for some time
>now?
>
Ben,
Not knowing where your peaks are eluting in the gradient makes it
difficult to tell if your peaks are coming from the same source, i.e. the
water. I had a similar problem which I fought for a long time. If you
really have "ghost peaks", they will elute in the position of the peaks
from your previous sample. Otherwise, if they persist with no sample
injection, then the buffers are the likely source. I tried many
commercially available bottled waters until I finally switched to the a
cartridge system for deionizing my water, whereupon the problem
disappeared. Hope this is of some help.
regards