Aleksander Swietlow
Ben Van Der Perre wrote:
> Dear ABRF'ers,
>
> We are having problems with 'ghost' -peaks that are appearing in are RPC
> runs. We are using a spheri-5 rp-18 column with a precolumn ( rp-8)
> The buffers are:
> A: 0.1M Triethylamine (TEA, >99%) in HPLC-grade water brought to pH7 with
> acetic acid (>99.8%)
> B: 80% acetonitrile in water
>
> INITIAL: 100% A
> LINEAR GRADIENT to: 50/50 A/B
> RINSE: 100% B
> END: 100%A
> We have tried many things to locate our problem. We changed the buffers,
> the columns but still there is a clear peak ones the gradient is started.
> It seems that this is a component that is present in the buffer A because
> the peak increases with the volume of buffer A that was pumped over the
> column. Could the stability of the triethylamine be given problems and
> should we buy a new bottle since the present one is in use for some time
> now?
>
> Kind regards
>
> Ben Van der Perre
> benvdp@innogenetics.be
> Immunochemistry
> Innogenetics.
-- Dr Aleksander Swietlow Polypeptide Laboratories (Sweden) AB Box 30047, S-20061 Malmoe, SWEDEN phone: +46 40 361233 fax: +46 40 361380