Quantitative MS Analysis of Phospholipids

Manfred Raida (Manfred_Raida@compuserve.com)
Fri, 2 Oct 1998 03:08:54 -0400

Dear ABRFs,
I mailed this message once, but I did not find it again, maybe I made an
error.
A collegue from the nearby Medical University asked me to post the
following request:

Dear collegues!

I am trying to quantify phospholipids, espeacially phosphatidylcholin
(PC), phosphatidylglycerol (PG), phosphatidylinositol (PI) and
phosphatidylethanolamin (PE) from pulmonary surfactant on a Perkin
Elmer SCIEX API I MS. So far I am using it as an ESI-MS with
an internal standard and than centroiding the peaks for quantification.
I am looking for a more sophisticated way to perform this task
(with MacQuan or other software). Who is having experiences with
and solutions for this problem.

Sincerely

Gunnar Rau, MD [100.86997@germanynet.de]
Medical School Hannover
Department of Pulmonary Pediatricc
Surfactant Lab
30623 Hannover
Germany

Thank you for help.
*************************************************
Manfred Raida
Niedersaechsisches Institut fuer Peptid-Forschung
(IPF)
Dep. of Analytical Peptide-Chemistry
Feodor-Lynen Strasse 31
D-30625 Hannover
Germany
E-Mail Manfred_Raida@compuserve.com
Tel.: +49 511 5466 210
Fax: +49 511 5466 102
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