Re: Quantitative MS Analysis of Phospholipids

Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Fri, 2 Oct 1998 13:43:49 +0000

If you are doing LC/MS, you might try quantitating the area under the peaks
as they elute from the LC. You can write a script to quantitate these
areas in multiple samples. We can give you a script we use to quantitate
phosphopeptides, which you can adapt.

Katheryn Resing

>Dear ABRFs,
>I mailed this message once, but I did not find it again, maybe I made an
>error.
>A collegue from the nearby Medical University asked me to post the
>following request:
>
>Dear collegues!
>
>I am trying to quantify phospholipids, espeacially phosphatidylcholin
>(PC), phosphatidylglycerol (PG), phosphatidylinositol (PI) and
>phosphatidylethanolamin (PE) from pulmonary surfactant on a Perkin
>Elmer SCIEX API I MS. So far I am using it as an ESI-MS with
>an internal standard and than centroiding the peaks for quantification.
> I am looking for a more sophisticated way to perform this task
>(with MacQuan or other software). Who is having experiences with
>and solutions for this problem.
>
>Sincerely
>
>Gunnar Rau, MD [100.86997@germanynet.de]
>Medical School Hannover
>Department of Pulmonary Pediatricc
>Surfactant Lab
>30623 Hannover
>Germany
>
>Thank you for help.
>*************************************************
>Manfred Raida
>Niedersaechsisches Institut fuer Peptid-Forschung
>(IPF)
>Dep. of Analytical Peptide-Chemistry
>Feodor-Lynen Strasse 31
>D-30625 Hannover
>Germany
>E-Mail Manfred_Raida@compuserve.com
>Tel.: +49 511 5466 210
>Fax: +49 511 5466 102
>**************************************************