Gramicidin purification

Laurent Boyer (lboyer@bgumail.bgu.ac.il)
Thu, 8 Oct 1998 11:58:42 +0200 (IST)

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Hello everybody,
I need to purify some Gramicidin D (from SIGMA no G-5002) : I would like
to separate the different isoforms by RPHPLC (with a C4 column).
I already tried with ACN/H2O and MeOH/H2O as mobile phase but
unsuccessfully in both cases : No signal after column !! the peptide's
maybe stuck into my column (?).
Any suggestions (eluents, gradients, sample solvent...)?

Thanking you in advance for your help,
LAurent.

Next message: Bao-Shiang Lee: "Gel of a peptide"
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