pepsyn: ellman's test

Wilhelm, Randy R (Randy.Wilhelm@mkg.com)
Mon, 12 Oct 1998 11:35:06 -0500

I am new to the area of peptide synthesis and have seen mention of Ellman's
reagent and Ellman's test to determine if disulfide formation is complete.
I have just obtained Ellman's original paper (Arch. Biochem. Biophys. 82,
70-77 (1959)). I am wondering how people actually use this. Could you
share your procedures? Do you use standards, such as cysteine or just use
the molar extinction coefficient of DTNB? Do some just use visual exam?

Thanks for your help!

Randy Wilhelm
Discovery Research / Peptide Group
Mallinckrodt, Inc.
St. Louis, MO
rrwilhe@mkg.com